Two distinct conformers of PrPD type 1 of sporadic Creutzfeldt–Jakob disease with codon 129VV genotype faithfully propagate in vivo

Table 1 Transmission features of sCJDVV resPrP^D to Tg129V and Tg129M mice

resPrP^D: proteinase K (PK)-resistant PrP^D; dpi: days post-inoculation (mean value ± standard deviation). Tohoku-2 positive (To-2 +) or negative (To-2 −) immunoreactivity. VV1²¹⁻²⁰*: sCJDVV1-2 with T2 accounting for ~ 5% of total resPrP^D. Dpi (1st pass.), Tg129V: VV2 vs. VV1²⁰, P < 0.0008; VV2 vs. VV1²¹, P < 0.04; VV1²⁰ vs. VV1²¹ or VV1²¹⁻²⁰*, P > 0.05. Dpi (1st pass.), Tg129M: VV2 vs. VV1²⁰, P < 0.03; VV2 vs. VV1^21–20* and VV1²⁰ vs. VV1²¹⁻²⁰*, P > 0.05; Dpi (2nd pass.), Tg129M: VV1²⁰ vs. VV1²¹⁻²⁰*, P < 0.009. Dpi, Tg129M: 1st vs. 2nd passage of VV1²⁰ or VV1²¹⁻²⁰*, P < 0.0001. Statistical significance was determined by one-way ANOVA and Student’s t-test. No transmis.: no transmission; pass.: passage; ND: not done

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