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Table 1 Transmission features of sCJDVV resPrPD to Tg129V and Tg129M mice

From: Two distinct conformers of PrPD type 1 of sporadic Creutzfeldt–Jakob disease with codon 129VV genotype faithfully propagate in vivo

Tg129V (1st pass.)
Inoculum VV120 VV121 VV121−20* VV2
Attack rate (%) 100 100 100 100
Incubation (dpi) 351 ± 16 425 ± 66 315 ± 66 215 ± 18
resPrPD replicated T120 (To-2 -) T121 (To-2 -) T121 (To-2 +) T2 (To-2 +)
Tg129M (1st pass.)
Inoculum VV120 VV121 VV121−20* VV2
Attack rate (%) 100 0 100 100
Incubation (dpi) 554 ± 53 0 570 ± 60 626 ± 56
resPrPD replicated T121−20 (To-2 -) No transmis T121−20 (To-2 +) T120 (To-2 -)
Tg129M (2nd pass.)
Inoculum VV120 VV121 VV121−20* ND
Attack rate (%) 100 0 100 ND
Incubation (dpi) 338 ± 30 0 292 ± 16 ND
resPrPD replicated T121−20 (To-2: ND) No transmis T121−20 (To-2: ND) ND
  1. resPrPD: proteinase K (PK)-resistant PrPD; dpi: days post-inoculation (mean value ± standard deviation). Tohoku-2 positive (To-2 +) or negative (To-2 −) immunoreactivity. VV121−20*: sCJDVV1-2 with T2 accounting for ~ 5% of total resPrPD. Dpi (1st pass.), Tg129V: VV2 vs. VV120, P < 0.0008; VV2 vs. VV121, P < 0.04; VV120 vs. VV121 or VV121−20*, P > 0.05. Dpi (1st pass.), Tg129M: VV2 vs. VV120, P < 0.03; VV2 vs. VV121–20* and VV120 vs. VV121−20*, P > 0.05; Dpi (2nd pass.), Tg129M: VV120 vs. VV121−20*, P < 0.009. Dpi, Tg129M: 1st vs. 2nd passage of VV120 or VV121−20*, P < 0.0001. Statistical significance was determined by one-way ANOVA and Student’s t-test. No transmis.: no transmission; pass.: passage; ND: not done