Fig. 4

Intramuscular injection of α-Syn PFF prompt pathological aggregation of α-Syn in neurons of the lumbar spinal cord, midbrain periaqueductal grey and thalamus. a Panoramic views: pSer129-α-Syn (in green) co-detection with neuronal nuclei antigen (NeuN, in red) in vehicle- (PBS, pH 7.4, left panel) and PFF-injected mice (right panel) lumbar spinal cord sections at 45 dpi (DH, dorsal horn; VH, ventral horn), b pSer129-α-Syn (in green) co-detection with neuronal nuclei antigen (NeuN, in red) in α-Syn PFF- injected mice. c Astrogliosis in the lumbar spinal cord, midbrain periaqueductal grey and thalamus was observed in the PFF-injected cohort. pSer129-α-Syn (in green) and glial fibrillary acidic protein marker (GFAP, in red) immunoreactivity, VH and DH of lumbar spinal cord, d midbrain periaqueductual grey (MB-PAG) and e thalamus. DAPI (blue) was used to stain the nuclei. Scale bar = 100 µm; insets in merge show 63X magnified views