Fig. 2

Reduction of Nlgn1 levels in temporal cortex of sporadic and familial AD cases. a Representative Western blot and relative GAPDH signal. Fifteen µg proteins per well of TBS soluble fractions of patients with sAD (n = 9), fAD (n = 9), PA (n = 15) and healthy Ctrl (n = 7) were separated by SDS-PAGE (4–12%) under reducing conditions. Neuropathological classification was based on Thal phases, Braak staging and CERAD score. A calibration curve was created using recombinant Nlgn1 extracellular domain fusion protein, to estimate the amount of Nlgn1 present in the samples. Samples were run on duplicate blots. b The box plots present the median and the interquartile range, the whiskers show the minimum and the maximum values. Significance: p ≤ 0.05. WB = Western blot