Fig. 2

Gene expression profiling data obtained after RNA sequencing of CNS-PNET cohort (n = 54). a. Heat-map of unsupervised hierarchical cluster analysis based on 500 differentially expressed genes disclosed that CNS_NBL (blue) were clustered separately from EPN_RELA (red); GBM_G34 (brown), and GBM_MYCN (orange) (Row Z-Scores: green: from 0 to − 6; red: from 0 to 6). Scales of principal component analysis (PCA); b. and two-dimensional t-distributed stochastic neighbor embedding (tSNE); c. analyses also revealed a separate distribution of CNS_NBL from other molecular groups