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Fig. 7 | Acta Neuropathologica Communications

Fig. 7

From: Pre-clinical characterisation of E2814, a high-affinity antibody targeting the microtubule-binding repeat domain of tau for passive immunotherapy in Alzheimer’s disease

Fig. 7

7G6 efficacy in an in vivo model of tau seeding and transmission. Full-length P301S tau seeds were injected into the left hippocampus of P301S transgenic mice pretreated with either IgG control (n = 11) or 7G6 antibody (n = 11) at a dose of 40 mg/kg i.p. Animals then received the same dose of antibody or vehicle once per week for a further 3 weeks until sacrifice. Vehicle-treated animals receiving no seed (n = 6) were included as an additional control group. Both hippocampi from each brain were extracted and separately treated with sarkosyl. Tau levels were then quantified in the sarkosyl-insoluble fraction from each sample by western blot and plotted for either the ipsilateral (a) or contralateral (b) hippocampi for each animal. Data are expressed as mean ± SEM and further analysed using a one-way ANOVA followed by Fisher’s LSD test. **** p ≤ 0.0001 IgG versus no seed control, * p ≤ 0.05 7G6 versus IgG for contralateral hippocampus, n.s. not significant

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