Single-nucleus RNA-seq identifies Huntington disease astrocyte states

Table 1 HD and Control Patient Data

Patient	Age	Gender	CAG Repeats	Other Neuropathology	Cause of Death	RNASeq	PMI^COLD	PMI^FROZEN
HD5493	48	F	46–25	Glial scars, dentate, cerebellum, small	PE, pneumonia	SN + BULK	N/A	9:33
HD5575	71	M	44–18	AA, H/I acute	Cardiac Arrest	SN + BULK	0:07	15:04
HD5301	54	M	46–19	AA, H/I acute	N/A	BULK	1:27	18:52
HD4929	50	M	49–16	H/I acute	Inanition	BULK	0:05	43:08
HD3859	36	M	50–17	NS	N/A	BULK	0:40	15.:00
HD5300	53	F	46–22	NS	Pneumonia	BULK	0:58	20:03
C5382	62	M		AA	Acute,PE	SN + BULK	N/A	5:24
C5404	54	F		AA	Acute, MI	SN + BULK	6:41	16:36
C4812	36	M		H/I acute	Acute, MI	BULK	N/A	8:37
C180	52	F		AA, H/I acute, infarcts, C, precentral gyrus	N/A	BULK	2:25	6:07
C98	55	M		AA	Acute collapse	BULK	6:05	37:00
C4518	56	M		AA, focal, old hem & infarcts, thalamus, stem, basal ganglia	Acute cardiac arrest	BULK	N/A	8:50

PE Pulmonary embolism, MI Myocardial infarction, AA Athero-arteriosclerosis, NS No other significant neuropathology, H/I Hypoxic/ischemic changes, C Caudate, N/A Not available. PMI^COLD hours between death and preservation of brain on ice. PMI^FROZEN hours between preservation on ice and freezing. Note that some of these patients died outside of Columbia University Medical Center and brains were sent here on ice. Cause of death was not available for some patients who died outside the medical center and whose autopsy was limited to the brain

ISSN: 2051-5960