Fig. 3
From: Protein disulfide isomerase ERp57 protects early muscle denervation in experimental ALS
ERp57 overexpression improves hindlimb electrophysiology in mutant SOD1 mice. a Example of electrophysiological recordings of compound muscle action potential (CMAP) in gastrocnemius muscle of non-Tg and SOD1G93A mice at post-natal day 90. Grey area shows CMAP signal after stimulation (first peak). b CMAP amplitude during early-symptomatic window. Statistical analysis was performed using two-way ANOVA with Tukey’s multiple comparison test. Mean ± S.E. is shown; p values for comparison between SOD1G93A and SOD1G93A/ERp57WT are shown: (“G93A” and “WT” in super index) *, p ≤ 0.05 (n = 3–7 animals per genotype). c Representative immunofluorescence micrographs of lumbar spinal cord sections stained with anti-ChAT and anti-MMP9 antibody at post-natal day 70. Ventral horns are shown. Scale bar: 50 μm. d Analysis of ChAT+/MMP9+ vulnerable motoneurons number in lumbar spinal cord quantified from at least 4 serial sections per animal. Statistical analysis was performed using one-way ANOVA with Tukey’s multiple comparison test. Mean ± S.E. is shown; p values: n.s., p > 0.05; *, p ≤ 0.05 (n = 3–7 animals per genotype)