Fig. 2

TDP-43 pathology distribution in different regions in TDP-43 PFFs-injected mice. Representative photomicrographs of p409-410 IHC staining in coronal brain sections of cortex (a–d), internal capsule (IC, e–h), midbrain (i–k), medulla oblongata (l–n), The seventh cervical spinal cord (C7, o–q), The third lumber spinal cord (L3, r–t) from TDP-43 M1-C mice analyzed at 8 mpi (n = 3 mice/group) in the ipsilateral (Ipsi) and contralateral (Contra) side of injection. In the cortex, p409–410 staining was mainly detected in the fifth layer of the ipsilateral primary motor cortex (M1, a, c) and contralateral M1 (b, d). e–h Show p409–410 staining in the Ipsi IC (e, g) and Contra IC (f, h). In the midbrain, i–k show p409-410 staining in the Ipsila red nucleus (RN, j), Contra RN (k) and bilateral substantia nigra (SN). In the medulla oblongata, l–n show p409–410 staining in the Ipsi decussatio pyramidum (py, m), Contra py (n) and inferior olive nucleus (ION). o–q show p409–410 staining in the Ipsi anterior horn of C7 (p) and Contra anterior horn of C7 (q). r–t show p409–410 staining in the Ipsila anterior horn of L3 (s) and Contra anterior horn of L3 (t). [Scale bars, a–b, 200 µm; c–d, p–q, s–t, j–k 50 µm; e–f, 100 µm; g–h, m–n, 20 µm; i, l, o, r, 500 µm]