Fig. 3

AAV-mediated 14-3-3θ overexpression delays αsyn inclusion formation in the substantia nigra. a Representative images of immunohistochemistry for GFP (blue) and TH (brown) in brain sections containing the SN from AAV-GFP mice and AAV-14-3-3θ/GFP mice injected with αsyn monomers. Colocalization of GFP and TH staining indicates viral induction into dopaminergic nigral neurons in the targeted area. Scale bar = 100 μm for 10 × image; scale bar = 25 μm for inset. b Representative images of pS129-αsyn immunostaining in AAV-GFP mice and AAV-14-3-3θ/GFP mice injected with PFFs at 3 mpi. Scale bar = 100 μm. c Representative images of pS129-αsyn immunostaining in AAV-GFP mice and AAV-14-3-3θ/GFP mice injected with PFFs at 6 mpi. Scale bar = 100 μm. d AAV-mediated 14-3-3θ expression in PFF-injected mice decreases pS129-αsyn positive inclusion counts at 3 mpi and increases counts at 6 mpi in the SN. Quantification of nigral inclusions at 3 mpi and 6 mpi in AAV-GFP mice and AAV-14-3-3θ/GFP mice injected with PFFs. n = 13–14 per group. *p < 0.05 (Student’s t-test). Error bars represent SEM. e Representative images of PFF-injected AAV-14-3-3θ mice stained for pS129-αsyn and either NeuN or GFAP demonstrate that αsyn inclusions are primarily associated with neurons. Scale bar = 10 μm