Fig. 4

Administration of fasudil hydrochloride increases IPAD in the hippocampus, but not cortex of denervated mice. a–f Photomicrographs showing the distribution of human Aβ40-AF555 (red) at 5 min post-injection into the hippocampus (a and b) and at 2.5 min after injection into the cortex (d and e) of control mice at baseline (a and d) and after administration of fasudil hydrochloride (b and e). The cerebrovascular basement membrane was labelled with anti-laminin (blue) and smooth muscle cells were identified with anti-α smooth muscle actin (green). Quantification of the total number of Aβ40-containing vessels in the hippocampus (c) and cortex (f) of control (con) and saporin (sap)-treated mice at baseline (n = 14–16 for hippocampus and n = 7–8 for cortex) and after fasudil hydrochloride (+F) (n = 5/group for both regions). g and h Photomicrographs of laminin staining in the hippocampus (g) and cortex (h) of control (upper panels) and saporin-treated mice (lower panels). i and j Quantification of % area covered by laminin in the hippocampus (i) and cortex (j) of control and saporin animals. n = 5–7/group. Scale bars = 100 μm. *p < 0.05, **p < 0.01, ***p < 0.001