Fig. 1

Saporin administration kills cholinergic neurons and fibres in wildtype mice. a–f Photomicrographs of ChAT staining in the medial septum and diagonal band of Broca (a and d), hippocampus (b and e) and cortex (c and f) in control (a–c) and mu-saporin treated C57BL/6 mice (d–f). (g-i), Quantification of % area covered by ChAT-positive neurons in the medial septum (g) and fibres in the hippocampus (h) and cortex (i). Scale bar = 100 μm. n = 5/group, *p < 0.05, **p < 0.01