Fig. 1

Evolution of experimental osmotic demyelinated lesions in the corpus striatum. a Representative micrographs of LFB/PAS-stained ODS lesions at different time points after the correction of serum sodium levels. The dashed line marks the lesion border, the dotted line indicates an already remyelinated area, indicated by pale LFB-staining, at 21 dpc; magnification × 20, scale bar 500 µm. b Demyelinated area as percentage of whole striatum (one dot represents one animal, mean ± SEM, one-way ANOVA and Tukey’s multiple comparison, *p < 0.05). c Percentage of myelinated axons per viewing field at different time points. Fiber tracts in the lesion center were analyzed (one dot represents one animal, mean ± SEM, one-way ANOVA and Tukey’s multiple comparison, p < 0.0001; **p < 0.01, ***p < 0.001). d Representative electron micrographs of transverse sections of striatal fiber tracts showing intralesional fibers at 3, 7, 14, 21 dpc, as well as perilesion fibers at 21 dpc and control animals. magnification × 3150. e g-ratios at 21dpc lesion versus perilesion (n = 5, one dot represents one axon, mean ± SD, t-test, p < 0.0001***)