Fig. 1

Behavioural assessment of sham and CON cohorts: a LRR time of sham (n = 14) and CON (all 3 CON cohorts, n = 29), two-tailed Mann–Whitney test. Lines showing median and interquartile range. b NSS of experimental cohorts at 30 min post-concussion and on imaging days: sham (n = 14), CON day 2 (n = 9), CON day 7 (n = 10), and CON day 14 (n = 10). Sham versus all CONs at 30 min: two-tailed Mann–Whitney test. Intra-cohort, 30 min versus imaging day: two-tailed Wilcoxon matched-pairs signed rank test. Inter-cohort NSS on imaging day: Kruskal–Wallis One-Way ANOVA with FDR-corrected (q < 0.05, two-tailed) post hoc tests. Data point displayed as mean and standard errors of means. c Proportion of time spent in each of four annuli in Open Field Test. Repeated Measures Two-way ANOVA with Geisser–Greenhouse correction and FDR-corrected (q < 0.05, two-tailed) post hoc tests. d Thigmotaxis Index of each cohort quantified from Open Field Test on imaging day. Brown–Forsythe and Welch One-way ANOVA with FDR-corrected (q < 0.05, two-tailed) post hoc tests. *P value < 0.05, **P value < 0.01, ***P value < 0.001