Fig. 5

Oncolytic adenovirus targets glioblastoma-associated FAP+ cells in vitro and vivo. a Animal experiment schema. b Immunofluorescence of FAP+ in 005 murine GBM after treatment with PBS, G47∆, and ICOVIR15. Quantification of FAP+ cells on the right. *p < 0.05 (t test). c, d Double immunofluorescence of FAP and adenovirus marker hexon (c) and GFP (005 marker) and hexon (d) after injections of ICOVIR15. Arrows point to representative double positive cells. e Hexon positivity (%) in FAP+ cells and GFP+ cells. f Cell growth/kill curves of FAP+ cells (left) and GFP+ 005 cells (right) after treating 005 tumor-derived acutely dissociated cells with mock (control) or ICOVIR15 (virus) in vitro. Representative microscopic images on day 7 are presented on top. Scale bar: 100 μm. *p < 0.05; **p < 0.01 (t test). g Immunofluorescence of FAP (red) and Hexon (blue), and GFP (green) and hexon (blue) during the time course following in vitro infection of 005 tumor dissociated cells with ICOVIR15. Scale bar: 100 μm. h Change in the fraction of hexon+ cells (%) within GFP+ 005 cells and FAP+ cells post-infection with ICOVIR15 in vitro. Error bars, SD