Fig. 5
Gender influences the extent of RGC dendritic impairment at 12 months in the 3xTg-AD and APPNL-G-F mice. a–i Sholl analysis of reconstructed RGCs from male and female mice compared to B6/J Control and within each model. a represents all the male data, d represents all the female data, b–c, and e–i compare single models to show significance. Two-tailed Mann–Whitney U test at each interval distance, *p < 0.05. Sholl analysis line represents mean of group at each interval, shaded error zones represent ± SEM. j Area under the Sholl curve. Two-way ANOVA: Interaction: F (2, 332) = 3.629, p = 0.0276; Genotype: F (2, 332) = 42.38, p < 0.0001; Gender: F (1, 332) = 5.701, p = 0.0175. k Maximum number of Sholl intersections. Two-way ANOVA: Interaction: F (2, 332) = 2.490, p = 0.0845; Genotype: F (2, 332) = 32.72, p < 0.0001; Gender: F (1, 332) = 4.182, p = 0.0416. l Total length of all dendrites. Two-way ANOVA: Interaction: F (2, 332) = 3.213, p = 0.0415; Genotype: F (2, 332) = 47.40, p < 0.0001; Gender: F (1, 332) = 6.137, p = 0.0137. Column Scatterplot j–l data points represent individual cells and error bars correspond to SD. B6J Control male: n = 64 cells (5 mice), B6J Control female: n = 50 cells (5 mice); 3xTg-AD male: n = 69 cells (6 mice), 3xTg-AD female: n = 75 cells (6 mice); APPNL-G-F male: n = 45 cells (3 mice), APPNL-G-F female: n = 35 cells (3 mice). All mice aged 12 months. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001. AUC area under the Sholl curve