Fig. 3

G34V mutation does not affect global H3K36me3. Increased H3K36me3 is observed at loci with G34V knockdown. a Relative proportion of H3K36me3 enrichment across gene elements with H3.3G34V expression or knock-down. b H3K36me3 enrichment at the NLGN2 gene is higher in KNS42 after G34V knockdown, compared to KNS42 no DOX control and non-transduced KNS42. However, transduced H3.3G34V into NHAs had no effect on H3K36me3 enrichment patterns. c Metagene profile of H3K36me3 enrichment. No significant difference in H3K36me3 enrichment was observed at the 1000 most H3K36me3 enriched loci (top left), nor at the 1000 most H3.3G34V enriched loci (top right) with or without DOX-induced H3.3G34V knockdown. No difference H3K36me3 enrichment was also observed at the 1000 loci with the greatest difference in H3.3G34V enrichment between KNS42 no DOX and DOX-induced H3.3G34V knockdown (bottom left). In contrast, greater H3K36me3 enrichment was observed in 1000 loci with the greatest difference in H3K36me3 enrichment with H3.3G34V knockdown, compared to no DOX control (bottom right). d Heatmap profiles of K36me3 peaks at loci with the top 1000 most difference in enrichment before and after knockdown. Signals from (left) KNS42 with DOX-induced G34V knockdown, (middle) KNS42 without G34V knockdown (no DOX control), and (left) the difference between them (Δ, left minus middle). Positive values indicate that KNS42 with DOX-induced G34V knockdown has higher K36me3 enrichment compared to no DOX control