Fig. 5

Dietary supplementation with D-PUFAs reestablishes the levels of mitochondrial dynamic- and axonal transport-related proteins in rats overexpressing α-syn. a Confocal microscopy images of midbrain sections acquired at 40 × and processed for colocalization analysis between TH⁺ (blue), Mfn2 (green) and Opa1 (red). b Mfn2 immunofluorescence signal. c Opa1 immunoreactivity. d Fluorescence micrographs of SN sections obtained at 40 × and labeled using antibodies against TH⁺ (blue), Drp1 (green) and human α-syn (red). e Protein expression profile of Drp1. Scale bar: 20 μm. Each value represents the mean ± SEM of three sections per animal. Four animals per group. f Representative blots of fractions stained with KLC-1 and DYNLT3 antibodies. Quantitative analysis of KLC-1 (g) and DYNLT3 (h) protein levels. Data are expressed as mean ± SEM and represented three rats per group. ****p < 0.0001, **p < 0.01 and *p < 0.05. Two-way ANOVA followed by Tukey’s post-hoc multiple comparisons test