Fig. 4
From: Clusterin ameliorates tau pathology in vivo by inhibiting fibril formation
CLU binds to tau and prevents tau aggregation. a In vitro tau fibrilization assay was performed with the 4R0N tau isoform in the presence or absence of recombinant human CLU and thioflavine T intensity was monitored during 24-h time course. b Quantification of thioflavine T signal at the 24-h time point. Four replicates were used. Data present as mean ± S.E.M. and analyzed by Student’s t test ***p < 0.001. c Western blot analysis of soluble (supernatant, s) and insoluble (pellet, p) fractions following tau fibril assembly in the presence or absence of exogenous CLU. d Tau assembly assay performed with 4R0N recombinant tau, tau PHFs from AD brains, and increasing concentrations of recombinant CLU. Four replicates were used. Data present as mean ± S.E.M. and analyzed by one-way ANOVA, **p < 0.01, ***p < 0.001, ****p < 0.0001