Fig. 5

Coronal overview of field RABV-infected mouse head sections after i.c. inoculation. a, b Maximum z- (a) and 3D projection (b) of mouse head sections after intracranial infection with field RABV [1.26x magnification; z = 1606 µm]. Indirect immunofluorescence staining against RABV P (red), NEFM (green), and nuclei (blue). Green fluorescence for NEFM not shown as separation from green autofluorescence was not possible at low resolution. Scale bar: 2000 µm. c, e, g, i Maximum z-projections of details from A (white boxes) at a magnification of 12.6x. Scale bar: 200 µm. d, f, h, j Confocal high-resolution z-stacks (corresponding to the indicated regions in c, e, g, i, respectively) [z = 35 µm (d), 32 µm (f), 36 µm (h), 40 µm (j); scale bar: 50 µm and 15 µm in detail]. Note: due to the separate confocal image acquisition, the orientations differ from the light sheet microscopy images in c, e, g, and i. c, d Infected eye, e, f nasal epithelium, g, h tongue, i, j and teeth in mouse head coronal section. k–n Maximum z-projection of serial mouse head sections (Additional file 1: Fig. S2B) [1.26x; z = 1780 µm (k), 1820 µm (l), 1606 µm (m), 1760 µm (n); green not shown]. Scale bar: 2000 µm