Table 1 Details of primary antibodies used for immunohistochemistry

Peanut agglutin lectin (PNA), biotinylated

#B-1075, Vector Laboratories, California, USA

1:3000

Retina, paraffin sections

20-min microwave, 10 mM citrate buffer, pH 6

Mouse anti-rhodopsin

#MAB5316; Sigma-Aldrich Pty. Ltd. NSW, Australia

1:600

Retina, paraffin sections

15-min microwave, 10 mM citrate,2 mM EDTA, 0.5% Tween 20, pH 6

Rabbit anti-RNA-binding protein with multiple splicing (RBPMS),

#ab194213; abcam, Victoria, Australia

1:250

Retina, flat mounts

None

Goat anti-calretinin

#AF5065; R&D Systems Minneapolis, MN, USA

1:1500

Retina, paraffin sections

1 mM EDTA, 0.05% Tween 20, pH8.0 15-min, microwave

Mouse anti-protein kinase C-alpha (PKC-α)

#ab31; abcam, Victoria, Australia

1:2000

Retina, paraffin sections

1 mM EDTA, 0.05% Tween 20, pH8.0 15-min, microwave

Mouse anti-calbindin

#C9848; Sigma-Aldrich Pty. Ltd. NSW, Australia

1:1000

Retina, paraffin sections

1 mM EDTA, 0.05% Tween 20, pH8.0 15-min, microwave

Mouse anti-lysosomal integral membrane protein 2 (LIMP2)

In-house; Hemsley et. al., 2008

1:500

Retina, paraffin sections

1 mM EDTA, 0.05% Tween 20, pH8.0 15-min, microwave

1:800

Brain, paraffin sections

Target Retrieval Solution (DAKO), 10- min microwave

Rabbit anti-glial gibrillary acidic protein (GFAP)

#Z033401-2; Agilent (DAKO), Santa Clara, CA, USA

1:500

Retina, paraffin sections

1 mM EDTA, 0.05% Tween 20, pH8.0 15-min, microwave

1:800

Brain, paraffin sections

10-min microwave, 10 mM citrate buffer, pH 6

Isolectin-B4-peroxidase conjugated

#L5391; Sigma-Aldrich Pty. Ltd. NSW, Australia

1:60

Brain and retina, paraffin sections

0.05% Trypsin, pH 7.6 15 min @ 37 °C