From: Is the eye a window to the brain in Sanfilippo syndrome?
Primary antibody/Lectin | Catalogue number, Source | Dilution | Tissue type | Pre-treatment |
---|---|---|---|---|
Peanut agglutin lectin (PNA), biotinylated | #B-1075, Vector Laboratories, California, USA | 1:3000 | Retina, paraffin sections | 20-min microwave, 10Â mM citrate buffer, pH 6 |
Mouse anti-rhodopsin | #MAB5316; Sigma-Aldrich Pty. Ltd. NSW, Australia | 1:600 | Retina, paraffin sections | 15-min microwave, 10Â mM citrate,2Â mM EDTA, 0.5% Tween 20, pH 6 |
Rabbit anti-RNA-binding protein with multiple splicing (RBPMS), | #ab194213; abcam, Victoria, Australia | 1:250 | Retina, flat mounts | None |
Goat anti-calretinin | #AF5065; R&D Systems Minneapolis, MN, USA | 1:1500 | Retina, paraffin sections | 1Â mM EDTA, 0.05% Tween 20, pH8.0 15-min, microwave |
Mouse anti-protein kinase C-alpha (PKC-α) | #ab31; abcam, Victoria, Australia | 1:2000 | Retina, paraffin sections | 1 mM EDTA, 0.05% Tween 20, pH8.0 15-min, microwave |
Mouse anti-calbindin | #C9848; Sigma-Aldrich Pty. Ltd. NSW, Australia | 1:1000 | Retina, paraffin sections | 1Â mM EDTA, 0.05% Tween 20, pH8.0 15-min, microwave |
Mouse anti-lysosomal integral membrane protein 2 (LIMP2) | In-house; Hemsley et. al., 2008 | 1:500 | Retina, paraffin sections | 1Â mM EDTA, 0.05% Tween 20, pH8.0 15-min, microwave |
 |  | 1:800 | Brain, paraffin sections | Target Retrieval Solution (DAKO), 10- min microwave |
Rabbit anti-glial gibrillary acidic protein (GFAP) | #Z033401-2; Agilent (DAKO), Santa Clara, CA, USA | 1:500 | Retina, paraffin sections | 1Â mM EDTA, 0.05% Tween 20, pH8.0 15-min, microwave |
 |  | 1:800 | Brain, paraffin sections | 10-min microwave, 10 mM citrate buffer, pH 6 |
Isolectin-B4-peroxidase conjugated | #L5391; Sigma-Aldrich Pty. Ltd. NSW, Australia | 1:60 | Brain and retina, paraffin sections | 0.05% Trypsin, pH 7.6 15 min @ 37 °C |