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Table 1 Details of primary antibodies used for immunohistochemistry

From: Is the eye a window to the brain in Sanfilippo syndrome?

Primary antibody/Lectin Catalogue number, Source Dilution Tissue type Pre-treatment
Peanut agglutin lectin (PNA), biotinylated #B-1075, Vector Laboratories, California, USA 1:3000 Retina, paraffin sections 20-min microwave, 10 mM citrate buffer, pH 6
Mouse anti-rhodopsin #MAB5316; Sigma-Aldrich Pty. Ltd. NSW, Australia 1:600 Retina, paraffin sections 15-min microwave, 10 mM citrate,2 mM EDTA, 0.5% Tween 20, pH 6
Rabbit anti-RNA-binding protein with multiple splicing (RBPMS), #ab194213; abcam, Victoria, Australia 1:250 Retina, flat mounts None
Goat anti-calretinin #AF5065; R&D Systems Minneapolis, MN, USA 1:1500 Retina, paraffin sections 1 mM EDTA, 0.05% Tween 20, pH8.0 15-min, microwave
Mouse anti-protein kinase C-alpha (PKC-α) #ab31; abcam, Victoria, Australia 1:2000 Retina, paraffin sections 1 mM EDTA, 0.05% Tween 20, pH8.0 15-min, microwave
Mouse anti-calbindin #C9848; Sigma-Aldrich Pty. Ltd. NSW, Australia 1:1000 Retina, paraffin sections 1 mM EDTA, 0.05% Tween 20, pH8.0 15-min, microwave
Mouse anti-lysosomal integral membrane protein 2 (LIMP2) In-house; Hemsley et. al., 2008 1:500 Retina, paraffin sections 1 mM EDTA, 0.05% Tween 20, pH8.0 15-min, microwave
   1:800 Brain, paraffin sections Target Retrieval Solution (DAKO), 10- min microwave
Rabbit anti-glial gibrillary acidic protein (GFAP) #Z033401-2; Agilent (DAKO), Santa Clara, CA, USA 1:500 Retina, paraffin sections 1 mM EDTA, 0.05% Tween 20, pH8.0 15-min, microwave
   1:800 Brain, paraffin sections 10-min microwave, 10 mM citrate buffer, pH 6
Isolectin-B4-peroxidase conjugated #L5391; Sigma-Aldrich Pty. Ltd. NSW, Australia 1:60 Brain and retina, paraffin sections 0.05% Trypsin, pH 7.6 15 min @ 37 °C
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