Fig. 4
From: In vivo RyR1 reduction in muscle triggers a core-like myopathy
Histological and immunofluorescent analyses show major defects in muscles. a TA Transversal section from CTRL and RyR1-Rec mice at D75 were stained with hematoxylin/eosin (H&E) and NADH. The mitochondria localization (NADH staining) is inhomogeneous in RyR1-Rec fibers, specifically in the small dark type I (slow) fibers with high mitochondria content (arrow head and inset). The nuclei (blue dots with H&E staining) are at the periphery of the fibers, and no evidence of regenerative fiber can be seen. Bar 50 µm. b Transversal TA sections from D75 CTRL and RyR1-Rec mice were stained with antibodies against RyR1 (green) and desmin (red). Bar 50 µm, and 10 µm in inset