Table 4 List of primer and probe sequences (5′–3′) used for real-time quantitative PCR (RT-qPCR)
From: RNA dependent suppression of C9orf72 ALS/FTD associated neurodegeneration by Matrin-3
α-tubulin (Drosophila) |  |
Primer 1 | ACCAGCCTGACCAACATG |
Primer 2 | CCTCGAAATCGTAGCTCTACAC |
Probe | /56FAM/TCACACGCG/ZEN/ACAAGGAAAATTCACAGA/3IABkFQ/ |
MATR3 (human) | Â |
Primer 1 | CTTCTTCTGTCTGCGTTCTTCT |
Primer 2 | TACTGTAAGCTGTGTTCACTCTT |
Probe | /56FAM/ACTCATTGC/ZEN/AGCAGCCTTCCTCA/3IABkFQ/ |
GAPDH (human) | Â |
Primer 1 | GTGGAGTCATACTGGAACATGTAG |
Primer 2 | AATGGTGAAGGTCGGTGTG |
Probe | /56-FAM/TGCAAATGG/ZEN/CAGCCCTGGTG/3IABkFQ/ |
Dendra2 | Â |
Primer 1 | ACTTCAAGCAGAGCTTCCC |
Primer 2 | ACGTTCTGGAAGAAGCAGTC |
Probe | /56-FAM/CAGATGCCC/ZEN/TTGTCCTCGAAGGTC/3IABkFQ/ |
GFP | Â |
Primer 1 | GAACCGCATCGAGCTGAA |
Primer 2 | TGCTTGTCGGCCATGATATAG |
Probe | /56-FAM/ATCGACTTC/ZEN/AAGGAGGACGGCAAC/3IABkFQ/ |
HIST1H3D | Â |
Primer 1 | GGACTTGCGAGCAGTCTG |
Primer 2 | CATCCAGGCTGTACTGCT |
Probe | /56-FAM/CCTTGGCCT/ZEN/TGTGGTGACTCTCA/3IABkFQ/ |