Fig. 8

Semi-quantitative western blotting analysis of human brain ATXN2. Brain homogenates from six normal controls and six FTLD-TDP cases were analyzed by western blotting, and each band intensity was quantified and statistically compared. a Immunoblots of each sample detected by the antibodies for ATXN2, RPS6, pTDP-43, and GAPDH. The blots were cropped for clarity and conciseness. b Semi-quantifications of band levels in each group were calculated and plotted. Data are mean ± standard deviation, and statistical comparisons were made by the Mann–Whitney U tests. The level of ATXN2 in FTLD-TDP cases was significantly decreased compared with controls (*P < 0.01). On the other hand, the levels of RPS6 and GAPDH were not largely changed between two groups. The bands of pTDP-43 in sarkosyl-insoluble fraction were found only in FTLD-TDP cases