Fig. 3

Colocalization images of ATXN2 in cytoplasmic organelle and related molecules in normal control brain. The confocal microscopic images of cytoplasmic ATXN2 and several organelle marker proteins, including endoplasmic reticulum marker Calnexin, ribosomal protein S6 (RPS6), Golgi Glycoprotein 1 (GLG1), Lysosome associated membrane protein 1 (LAMP1), ATXN2’s related protein poly-A binding protein 1 (PABP1) and TDP-43, are shown. a–f Representative fluorescent double-stained images from the upper layer of the cerebral temporal cortex, including merged images with DAPI staining and two-dimensional scatterplots. ATXN2 is partially localized within the endoplasmic reticulum (a) and is strongly associated with ribosomes (b) and PABP1 (e) in controls, while ATXN2 did not localize within the Golgi apparatus (c) and lysosomes (d). There was no colocalization between ATXN2 and TDP-43 (f). Pseudo coloring was used for each channel