Fig. 2

The extent of CMH-induced cerebrovascular leak is region-specific. a Frozen brain sections taken from mice exposed to 7 days hypoxia (8% O₂) were dual-labelled for CD31 (AlexaFluor-488) and fibrinogen (Cy-3). Images were captured in four different brain regions: brainstem (BS), olfactory bulb (OB), cerebral cortex (CX) and cerebellum (CB). Scale bar = 200 μm. b, c Quantification of the number of leaky (fibrinogen-positive) blood vessels/FOV (b) or total area of vascular leak/FOV (c) after 7 days hypoxia. Results are expressed as the mean ± SEM (n = 4 mice/group). *p < 0.05; **p < 0.01. Note that hypoxia-induced vascular leak was significantly higher in the brainstem and olfactory bulb versus the cerebral cortex and cerebellum