Fig. 4
From: Overexpression of UBQLN1 reduces neuropathology in the P497S UBQLN2 mouse model of ALS/FTD
UBQLN1 overexpression reduces deposition of UBQLN2 inclusions compared to the brains of P497S mutant mice. a Staining of representative sagittal sections of the dentate gyrus (DG) (a–h), CA1 (i–p) and cortex (q–x) regions of the brain for UBQLN2 and DAPI in the four genotypes at 52 weeks of age. Scale bars shown. b Quantification of the number of UBQLN2 inclusions between 1 and 10 μm in size counted in identical size regions of the DG, CA1 and cortex regions (2 males and 1 female for each genotype). **P < 0.01, ***P < 0.001, ****P < 0.0001. c Filter-trap assay showing detection of UBQLN2 inclusions in 50 μg of protein brain lysate analyzed for the four genotypes for animals at 52 weeks of age. Samples analyzed were: Non-Tg, 2 male (M) and 1 female (F); UBQLN1 all F; P497S, 1 M, 2 F; UBQLN1/P497S all M. d Quantification of the intensity of UBQLN2 immunoreactivity for the filter shown in c. **P < 0.01