Fig. 1

1000 repeat DPRs are stable in the Drosophila Genome at 12 months post-injection. a Southern blots showing DPR-EGFP constructs at the expected length relative to both positive controls (+) and predicted size. Lanes show ladder, two independent lines per genotype (1 and 2), negative controls (−, DNA from wild type flies) and positive controls (+, DNA from wild type flies spiked with 1000 repeat DNA). b Immunoblots showing DPR constructs are expressed within the Drosophila nervous system. DPRs were detected with either anti-GFP (AP1000, GA1000 and PR1000) or anti-GR. Asterisks show DPR bands