Fig. 2

DNA methylation clustering analysis of the 30 LGNET with FGFR1 alterations (triangles), alongside a reference set of CNS tumor samples generated at DKFZ (circles). Shown is a two-dimensional representation of pairwise sample correlations using the 32,000 most variably methylated probes by uniform manifold approximation and projection (UMAP). Five LGNET from this cohort did not definitively cluster with known reference classes and are colored gray (uLGNET #1–5). Reference methylation classes are: DLGNT, diffuse leptomeningeal glioneuronal tumor; DNT, dysembryoplastic neuroepithelial tumor; EVN, extraventricular neurocytoma; GG, ganglioglioma; LGG MYB, low-grade glioma with MYB or MYBL1 rearrangement; PA MID, midline pilocytic astrocytoma; PA PF, posterior fossa pilocytic astrocytoma; PA ST, supratentorial/hemispheric pilocytic astrocytoma; PXA, pleomorphic xanthoastrocytoma; RGNT, rosette-forming glioneuronal tumor. See Additional file 1: Table S5 for the list of reference samples from DKFZ used in the clustering analysis