Fig. 5

A53T SynGFP aggregates appear first in axons. a Live in vivo multiphoton 3D projection showing serial images of the same neuron at progressive time points after PFF injection. A53T SynGFP aggregates (white arrow) form first in the axon and predict formation of a somatic inclusion (yellow arrows) in this neuron. b Group data show the location of initial A53T SynGFP aggregate formation as a percentage of analyzed cells. The overwhelming majority of the time (555/703; 78.95%) the first evidence of SynGFP aggregation was in the axon alone. In a subset of cells (148/703; 21.05%) the first evidence of aggregation was in both the axon and the cell body, indicating that the initial aggregate formation was not captured at the specific imaging time point at which those cells were imaged. There was no evidence (0/703; 0.00%) that the initial site of aggregation was in the cell body. This is strongly suggests aggregation begins in axons (Chi-square (2) = 505.8, p < 0.0001, N = 703 cells)