Fig. 4

Double immunofluorescence. Panels a–i show anti-AT8 immunohistochemistry in the medullary reticular formation. Tau aggregations are more prominent in the cholinergic neurons of the nucleus ambiguus (a–c) than in TH-immunopositive cathecolaminergic neurons in the ventrolateral medullary nucleus (d–f) or TrOH-immunopositive serotonergic neurons in the raphe nucleus (g–i). Panels j–l demonstrate occasional immunopositivity of phosphorylated TDP-43 within neurons containing AT8-immunopositive inclusions of the hypoglossal nerve. Abbreviations: ChAT, choline acetyltransferase; N. ambiguus, nucleus ambiguus; N. hypoglossal nerve, nucleus of the hypoglossal nerve; N. raphe, raphe nucleus; TH, tyrosine hydroxylase; and TrOH, tryptophan hydroxylase. Scale bars: a–c 50 μm, d–i 100 μm, and j–l 10 μm