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Table 2 Immunohistochemistry details. Primary and secondary antibody, Avidin–Biotin Complex (ABC) and DAB details [22, 26, 43, 47, 48]

From: Assessment of radial glia in the frontal lobe of fetuses with Down syndrome

Antigen Target Species Dilution Cat number Source
Alpha-crystallin B chain (CRYAB) Apical (ventral/truncated) radial glia Anti-mouse 1:250 ab13496 abcam
Homeodomain-only protein (HOPX) Basal (outer) radial glia Anti-rabbit 1:250 11419-1-AP Proteintech
SRY (sex determining region Y)-box 2, (SOX2) Progenitor cell and radial glial marker Anti-rabbit 1:500 ab97959 abcam
Vimentin Intermediate filament protein—Radial glia fibres and cell body Anti-rabbit 1:500 M072501-2 DAKO
glial fibrillary acidic-protein (GFAP) Intermediate filament protein—Radial glia fibres and cell body Anti-rabbit 1:1000 G3893 Sigma
Biotinylated IgG Secondary antibody Horse anti-mouse 1:200 BA-2000 Vector Labs
Biotinylated IgG Secondary antibody Goat anti-rabbit 1:200 BA-1000 Vector Labs
Avidin-Biotin Complex–Horseradish Peroxidase (ABC-HRP)    1:200 PK-6200 Vector Labs
3,3’-diamino-benzidine (DAB)    1:10 34002 Thermo
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