Table 2 Immunohistochemistry details. Primary and secondary antibody, Avidin–Biotin Complex (ABC) and DAB details [22, 26, 43, 47, 48]
From: Assessment of radial glia in the frontal lobe of fetuses with Down syndrome
Antigen | Target | Species | Dilution | Cat number | Source |
|---|---|---|---|---|---|
Alpha-crystallin B chain (CRYAB) | Apical (ventral/truncated) radial glia | Anti-mouse | 1:250 | ab13496 | abcam |
Homeodomain-only protein (HOPX) | Basal (outer) radial glia | Anti-rabbit | 1:250 | 11419-1-AP | Proteintech |
SRY (sex determining region Y)-box 2, (SOX2) | Progenitor cell and radial glial marker | Anti-rabbit | 1:500 | ab97959 | abcam |
Vimentin | Intermediate filament protein—Radial glia fibres and cell body | Anti-rabbit | 1:500 | M072501-2 | DAKO |
glial fibrillary acidic-protein (GFAP) | Intermediate filament protein—Radial glia fibres and cell body | Anti-rabbit | 1:1000 | G3893 | Sigma |
Biotinylated IgG | Secondary antibody | Horse anti-mouse | 1:200 | BA-2000 | Vector Labs |
Biotinylated IgG | Secondary antibody | Goat anti-rabbit | 1:200 | BA-1000 | Vector Labs |
Avidin-Biotin Complex–Horseradish Peroxidase (ABC-HRP) |  |  | 1:200 | PK-6200 | Vector Labs |
3,3’-diamino-benzidine (DAB) |  |  | 1:10 | 34002 | Thermo |