Fig. 6

Microgliosis in the hippocampi of TgA-5xFAD mice. a Representative confocal micrographs from the dentate gyrus (DG) and subiculum immunolabelled for Aβ (anti-6E10) and microglial marker Iba1. Analyses b & c are from 5 individual mice with four sections being imaged per mouse at 40X magnification using confocal microscopy. Two images along the DG, one along the CA3, one along the CA1 and one in the subiculum were analyzed for number of plaques, number of microglia and number of microglia around plaques. b Quantification of the sum (average across sections and mice) number of microglia in contact with plaques across the whole hippocampus. c Quantification of the average number of microglia in contact with each plaque across all subfields. Scale bars = 50 μm. Data are presented as mean ± SEM. Experiments were analyzed using unpaired t-test. *p < 0.05. Raw data available at: https://doi.org/10.6084/m9.figshare.12115782