Fig. 3

IPAD in corpus callosum. (a-c) Distribution of amyloid-β in relation to collagen IV and smooth muscle actin. Amyloid-β (red) was observed along the white matter tracts (c & d) and co-localising (Pink) with collagen IV in the walls of capillaries (yellow arrows) and arterioles (white arrows). (e-h) An arteriole in the white matter - see box in (d) -showing Aβ in the tunica media. Merging of the blue collagen IV staining in the basement membranes in (e) with red amyloid (g) produces a pink colour of co-localisation in (h). (i-l) A leptomeningeal artery in the hippocampal fissure abutting on to the white matter shows Aβ co-localized (pink) with collagen IV in the tunica media (lower arrow) and in the adventitia (upper arrow). (m-p) shows red Aβ (arrow) in the wall of a white matter capillary that is also stained blue for collagen IV in the basement membrane. Scale bars a-d = 200 μm, e – h = 20 μm & i –p = 10 μm