Fig. 5

HEX co-expression prevents aSYN association with lipid compartments. a Illustration of the lipid extraction paradigm. Protein-lipid interactions may mask or modulate epitopes and alter detection by antibodies. Delipidation would extract these complexes and allow detection by conventional methods. b Representative immunoblot of aSYN17 monomer and HMW-aSYN in CTRL+aSYN or HEX+aSYN AAV6-injected rat SN, either untreated (−) or lipid extracted by heating at 65C for 18 h (+). c Densitometric quantifications of HMW-aSYN immunoblots from lipid extracted fractions (+) expressed as % of the lipid containing (−) fraction (*:p < 0.05, Welch’s t-test, n = 5 and 7 / group). d Quantifications as in (c) for aSYN17 monomer (n = 6 and 7 / group). Data represents median, 95% CI and total range with individual data points shown for all graphs. e Pearson’s correlation analysis of aSYN-HMW measurements as in (c) with SDS-soluble aSYN17 / GAPDH values from Fig. 4g (N = 10)