Fig. 1

ScFvMC1 detection and peripheral organs’ morphology. a Six-month old mice (JNPL3 shown) were IV-injected with saline, 100 μg of IRDye-labelled antibodies (scFvMC1-IR and MC1-IR) and unlabeled antibodies (scFvMC1-UNL and MC1-UNL). Two hours post-injection, brain homogenates from cortex (Ctx), hippocampus (Hip) and hindbrain (HB) were spotted on nitrocellulose and absorbance of the IRDye label measured. Saline and unlabeled antibodies showed no signal (Sapphire Biomolecular Imager, Azure Biosystems). b, c AAV1-CAG-scFvMC1 was injected in tibialis muscle of P301S or JNPL3 (JNPL3 shown), mice sacrificed 4 months later and peripheral organs harvested. Tissue lysates were analyzed for scFvMC1 expression with anti-Myc/tag antibody showing localized expression in the injected site (b, right tibialis, Controls = AAV1-eGFP) while other peripheral organs did not show scFv signal (c). ScFvMC1: MW around 30 kDa; tubulin is used as housekeeper: MW at 55 kDa. d Hematoxylin and eosin (H&E) staining was performed on kidney, liver, tibialis muscle and heart: representative images of each organs show no significant changes in morphology (Controls = AAV1-CAG-eGFP; Bright field microscope, scale bar: 300 μm)