Fig. 3

5’UTR G4C2 repeats with different carboxyl termini elicit differential toxicity in Drosophilaa Representative eye phenotypes from flies of the indicated genotypes crossed to GMR-GAL4 to drive expression in developing ommatidia (right) and quantification of eye phenotype scores (right). b The number of progeny carrying the transgene was determined after flies of the indicated genotypes were crossed to a ubiquitous driver (act5c-GAL4). c Quantification of the distance crawled by 3rd instar larvae from the crosses of the indicated fly genotypes to a larval motor neuron specific driver (OK6-GAL4). d Flies carrying the indicated transgenes were crossed to a Tubulin Geneswitch driver (Tub5) to allow ubiquitous expression post eclosion. Adult male flies were placed on food containing RU-486 to activate gene expression and their viability was tracked over 50 days. Graphs represent means ± SEM. ** p < 0.01 by Kruskal-Wallis after Dunn’s correction for multiple comparisons. Log-rank (Mantel-Cox) test for survival, * p < 0.05, ** p < 0.01. The number of flies for each genotype was > 100