Fig. 1

5xFAD exosomes are taken up by WT neurons in vivo. (a) A schematic diagram of the experimental design. Exosomes isolated from brains of 5xFAD mice were first labeled with lipid-binding dye Vybrant CM DiI before being injected intracranially into wild type (WT) mice. Forty-eight hours post-injection, mice were sacrificed, and brains were collected and prepared for cryo-sectioning. (b, c) Representative immunocytochemistry images of sections of brains injected with (b) WT exosomes and (c) 5xFAD exosomes showing that FAD exosomes are internalized by WT neurons, insert is a higher magnification of the selected ROI