Fig. 3

Fyn depletion reduced tau^P301L-induced abnormal tau hyperphosphorylation. a Using soluble brain lysates, uninjected WT and Fyn KO mice did not show any abnormal tau hyperphosphorylation at the pS199/pS202 or pY18 epitopes. (Dotted white lines in A and B indicated places where noncontiguous lanes were brought together from the same blot.) b Using soluble brain lysates, WT-AAV and Fyn KO-AAV mice specifically showed the presence of human tau (htau) as well as pS199/pS202 and pY18 epitopes. c Quantification for total tau normalized to GAPDH (left), pS199/pS202 normalized to total tau (center), and pY18 normalized to total tau [88]. Relative to WT-AA, Fyn KO-AAV showed decreased tau hyperphosphorylation at pS199/pS202 (19 WT-AAV and 7 Fyn KO-AAV mice used, p = 0.0106) and pY18 epitopes (12 WT-AAV and 7 Fyn KO-AAV mice used, p = 0.0168) while total tau expression was not significantly different between Fyn KO-AAV and WT-AAV (22 WT-AAV and 7 Fyn KO-AAV mice used, p = 0.0532). To calculate p values, nonparametric t test was used for total tau and unpaired parametric t-test was used for to pS199/pS202 and pY18