Fig. 6

Fyn Inhibition Prevents Tau Seeding in Neurons. (a) Representative blots of Tau extracts using HT7 and AT180 antibodies to show prominent Tau bands between 50 and 75kD from human AD patient as compared to a healthy control. (b) Immunostaining of endogenous mouse Tau and MAP 2 in WT neurons fixed with methanol at DIV21 after AD-Tau seeding and treatment at DIV7. Left panel shows neurons with no AD-Tau seeding nor treatment. Middle panel shows neurons with AD-Tau seeding and treatment with vehicle (water). Right panel shows neurons with AD-Tau seeding and treatment with 0.5 μM AZD0530. (c) Quantification of percentage of mouse tau-positive area within MAP 2-positive area. Neurons were either not seeded with Tau; only seeded with AD-Tau; seeded with AD-Tau and treated with water as vehicle; seeded with AD-Tau and 0.5 μM of AZD0530; or seeded with AD-Tau and 1 μM of AZD0530. The background signal from images of neurons without AD-Tau treatment was subtracted. Experiments were performed in triplicate. Each data point represents the average of values obtained from four images taken from one well. Data are mean ± SEM. n = 9–18 per experimental condition. **p < 0.01; One-way ANOVA with Dunnett’s multiple comparisons test. (d) Same quantification as C, except each data point represents the average of three wells of each condition from one experiment. Data are mean ± SEM. n = 3. *p < 0.05; Repeated measures one-way ANOVA with Dunnett’s multiple comparisons test