Fig. 4

Quantification of TIMP3-stained vessels in CAA-NH and CAA-ICH cases, classified according to staining grade. The percentage of leptomeningeal vessels with full TIMP3 staining did not differ between groups (a), whereas the percentage with partial staining was significantly lower in the CAA-ICH group (b). Serial sections stained for Aβ (c, e) and TIMP3 (d, f) in a CAA-NH case (c, d) and a CAA-ICH case with low levels of TIMP3 expression (e, f) showed that these proteins colocalize. Scale bar = 200 μm. Assessment of only fully Aβ-stained leptomeningeal vessels showed no difference in the percentage of vessels that had full TIMP3 staining (g), but a lower percentage of partially stained vessels in the CAA-ICH group (h). The observed differences were predominantly driven by the subgroup of CAA-ICH cases with low TIMP3 expression (open triangles). Plots indicate median values with interquartile range. CAA-NH = CAA-non haemorrhagic, CAA-ICH = CAA-related ICH, *p ≤ 0.05; ***p ≤ 0.001