Fig. 3

Conformational stability immunoassay (CSI) and proteinase K (PK) titration performed on “pure” sCJDMV2 variants, sCJDMV1 and sCJD subtypes used as controls. a coordinate graph showing the stability indexes ([GdnHCl]1/2, i.e., the concentration of GdnHCl corresponding to midpoint resPrPD denaturation) related to the individual MV2C and MV2K variants and the MV1 subtype, along with VV2, MM2 and MM1 used as sCJD subtype controls; in the MV2K variant, the stability index was determined separately for the 20 kDa and 19 kDa components, as well as combined (total). b coordinate graph of PK titration indexes (PK1/2, i.e., U/ml of PK corresponding to midpoint total PrP digestion) in MV2K (19 and 20 kDa components, separately), MV1 as well as VV2 and MM1 subtype controls. Both assays performed on total PrP. #: p < 0.0001; &: p < 0.0002 *p < 0.04 (See Additional File 1: Table S1 for index scores)