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Fig. 5 | Acta Neuropathologica Communications

Fig. 5

From: RBM45 associates with nuclear stress bodies and forms nuclear inclusions during chronic cellular stress and in neurodegenerative diseases

Fig. 5

RBM45 solubility during conditions of cellular stress. a Workflow to obtain soluble and insoluble total protein extracts from untreated and stressed HEK293 cells expressing endogenous, N-terminally FLAG-tagged RBM45. “SN” = supernatant. b Western blotting for FLAG-tagged RBM45 (α-FLAG), GAPDH (negative insoluble fraction control), and TDP-43 (positive insoluble fraction control) following sodium arsenite treatment (1 mM for 1 or 2 h). c FLAG-RBM45, GAPDH and TDP-43 solubility following heat shock (1 h at 42 °C)

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Acta Neuropathologica Communications

ISSN: 2051-5960

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