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Table 1 PrPSc abundance and prion seeding activity in CJD brain homogenate samples inoculated into transgenic mice

From: Transmission characteristics of heterozygous cases of Creutzfeldt-Jakob disease with variable abnormal prion protein allotypes

Case #aSubtypeaPrPSc (SpC)bSD50/gc
ControlMM1ND>  5 × 109
3MV1 + 2C280 ± 1035 × 109
4MV1 + 2C48 ± 99 × 107
9 CCMV2K + 2C56 ± 128.9 × 1010
9 CbCMV2K + 2C126 ± 152.8 × 1010
12MV2K + 2C90 ± 41.6 × 109
19 (iCJD)MV2K133 ± 193.5 × 1011
10MV2K175 ± 191.2 × 1011
20 (iCJD)MV2K171 + 441.6 × 1011
7MV2K581 ± 892.8 × 1011
18 (iCJD)MV2K65 ± 72.8 × 1011
11MV2C290 ± 93.4 × 1010
  1. aNeuropathological subtype of cases originally described in [28]. All cases are sCJD except where noted as iCJD. CC cerebral cortex, CbC cerebellar cortex
  2. bSpC spectral counts. Total number of PrPSc peptides as determined by mass spectrometry of phosphotungstic acid precipitated CJD brain material For mean + standard deviation in previous sentence, please underline the + sign. are adapted from [28]
  3. cSD50/g = prion seeding doses (SD) yielding 50% positive wells in replicate RT-QuIC reactions per 1 g of MV brain homogenate [45]. Values were calculated from 3 independent end-point titration experiments
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