Fig. 8
Spongiform change and PrPSc deposition differ in the brains of tg66 mice inoculated with two different brain regions from a single case of MV2K + 2C sCJD. a H&E staining of a tg66 mouse inoculated with MV2K + 2C sCJD case 9 CC (502 dpi, left column) or 9 CbC (481 dpi, right column). The black arrows indicate eosinophilic amyloid plaques. b PrPSc deposition in a tg66 mouse inoculated with MV2K + 2C sCJD case 9 CC (502 dpi, left column) or 9 CbC (481 dpi, right column). The black arrows indicate PrPSc-positive amyloid plaques. The fields are matched to those shown in panel a. Sections were stained using the mouse monoclonal anti-PrP antibody 3F4 conjugated to biotin. The mean percentage of PrPSc-M129 in each sample is given under the case number. Brain regions shown are indicated in the middle of the figure. For all panels, scale bar = 50 μm