Fig. 7
Different patterns of PrPSc deposition in the brains of tg66 mice inoculated with sporadic or iatrogenic cases of MV2K CJD. a PrPSc deposition in a tg66 mouse inoculated with MV2K sCJD case 7 (386 dpi). The black arrows indicate PrPSc-positive amyloid plaques. The fields are matched to those shown in Fig. 6a. PrPSc deposition in case 10 was identical to that of case 7. b PrPSc deposition in tg66 mice inoculated with MV2K iCJD cases 19 (185 dpi, left column) and 20 (458 dpi, right column). The fields are matched to those shown in Fig. 6b. PrPSc-positive amyloid plaques are not present but occasional plaque-like deposits of PrPSc were observed. PrPSc deposition in case 18 was the same as that of case 19. The mean percentage of PrPSc-M129 in each sample is given under the case number. All sections were stained using the mouse monoclonal anti-PrP antibody 3F4 conjugated to biotin. For all panels, scale bar = 50 μm