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Fig. 4 | Acta Neuropathologica Communications

Fig. 4

From: Ultrastructural and dynamic studies of the endosomal compartment in Down syndrome

Fig. 4

Confocal microscopy and TEM imaging of BFCNs from 4-month-old Ts65Dn mice. a Representative confocal images of DAPI (blue), ChAT (red), EEA1 (green) labelling and overlay in BFCNs from WT and Ts65Dn mice. For clarity, NeuN staining is not shown; b Quantification of EEA1-positive puncta surface at equatorial section of BFCNs shows a significant increase in mean area in Ts65Dn mice as compared to WT mice (mixed effects ANOVA, genotype *p-value = 0.04); c Low magnification micrograph of a ChAT gold-labelled BFCNs of a Ts65Dn mouse. Early endosomes are labelled by an EEA1-DAB precipitate. The soma profile is pseudo-colored in yellow for clarity. N indicates the nucleus and asterisks show two lamellar bodies, an organelle specifically found in cholinergic neurons. The squared zone is enlarged in (d) showing an isolated DAB-EEA1-labelled endosome and a cluster of at least 2 endosomes, the latter being magnified in (e). The pseudo-colored red halo in (e) suggests the extent of labelled area as it should appear with confocal analysis

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