Fig. 5
Effect of AQP4 deficiency on soluble Aβ levels in 5xFAD female mice. a and b The amounts of soluble Aβ42 (a) and Aβ40 (b) extracted from the cerebral hemispheres of 5xFAD (red columns) and 5xFAD/AQP4 KO (blue columns) mice were determined by ELISA. One individual 32-week-old 5xFAD female mouse showed more than 4.0 and 7.5 times higher soluble Aβ42 and Aβ40 concentrations, respectively, compared with the average of the other seven individuals of the same group. Therefore, this mouse was excluded from all analyses, including insoluble Aβ level shown in Fig. 4m and n. Values are mean ± S.E.M of 6–7 individuals. c-f Time course of ISF-soluble Aβ42 (c and d) and Aβ40 (e and f) concentrations in hippocampal region of 5xFAD (red circles) and 5xFAD/AQP4 KO (blue squares) females at 25 weeks (c and e) and 33 weeks (d and f) of ages measured by ELISA of samples obtained by microdialysis. Values are mean ± S.E.M of 6–9 individuals