Fig. 6

Neurotoxic effect of Aβ₄₂/astrosome complexes on primary neuronal cultures. Representative single-focal-plane images of β-tubulin and Tom-20 labeling obtained with control (a), Aβ₄₂ (b), astrosome (d), or Aβ₄₂/astrosome-incubated (e) primary cultured mouse neuron. Arrows indicate mitochondrial clusters. c Average normalized density of β-tubulin labeling reveals that the greatest loss occurs in cultures treated with Aβ₄₂/astrosome complexes. N = 6. One-way ANOVA with Student-Newman-Keuls (SNK) post hoc test). ***p < 0.001, **p < 0.005, *p < 0.05). f TUNEL assay detected a 2.6-fold increase in neuronal cell death when Aβ₄₂ and astrosomes were combined (N = 4. One-way ANOVA with SNK post hoc test)