Fig. 6
pT-ION-induced gene expression changes in the unilateral LHb and the downregulation of Tacr3 gene expression. (a) The volcano plot is based on the expression values of all genes detected by microarray. (b) The heat maps showing DEGs with fold change ≥2.0 (p < 0.05). s, sham group; i, nerve injury group. (c) GO annotations of DEGs in 20 selected biological processes. (d) KEGG pathway enrichment analysis of DEGs in the selected 20 pathways. (e) Gene co-expression network. Each circle corresponds to a protein-coding gene (mRNA). The red circles represent up-regulated genes, and the green circles represent down-regulated genes. The size of the circle represents the degree of correlation with other genes, and the stronger the correlation degree, the larger the circle. The color of the circle is determined by the log (fold change) of each gene. Lines ending with arrows and vertical lines represent positive and negative interactions between genes, respectively. (f) PPI String network. Circles and lines represent target genes and interactions between genes, respectively. The sizes of the circles represent the degree of correlation degree with other genes, and the stronger the degree of correlation, the larger the circle. The color of the circle is determined by the degree of correlation. (g) Ridge map and RT-qPCR validation of pT-ION-induced differential expression of the Tacr3 gene in the LHb. n = 4 samples (8 mice) per group. ** p < 0.01 vs. the sham group