Fig. 6

Appearance of additional gamma-tubulin+ foci of microtubule nucleation (upper panel) and immunoreactivity for Aurora A in arrested mitoses (lower panel). a, b Several foci with gamma-tubulin (γ-tub) immunoreactivity in the vicinity of metaphase chromosomes, some of which are misaligned (arrows in the insets). a’ and b’ – split (a and b) images, respectively. Insets show position of chromosomes. For an animation of 3D reconstruction of (a, b) see Additional files 6A,B. c, d Foci of NUMA immunoreactivity are distributed all over the area occupied by metaphase chromosomes, many of which are misaligned and only partly colocalize with gamma-tubulin. c and c’ – split (c and d) images, respectively. For an animation of 3D reconstruction of (c, d) see Additional files 6C, D. e-i Immunoreactivity for Aurora A in normal mitoses (e, h) and abnormal mitoses with misaligned chromosomes (f, g, i). Note that in normal mitosis Aurora A localizes predominantly in the centrosomes, whereas in mitoses with misaligned chromosomes, small foci of Aurora A immunoreactivity are distributed over a large volume around the chromosomes. Note in G the difference between arrested mitosis (arrowhead) and normal mitosis (arrow). Lower right insets show positions of the chromosomes. All images were obtained with confocal microscope. Scale bars: a,b,d,f,h,i = 5 μm, c = 3 μm, = 10 μm, g = 8 μm